Journal of Animal Reproduction and Biotechnology

Table. 5.

Table 5

Percentages of viable, intact plasma membrane and DNA of buck spermatozoa extended in Tris-egg yolk enriched with Annona muricata aqueous extract and stored at 4℃ for 72 hours

Parameter	Treatment	Storage duration (h)

		6	24	48	72
Viability (%)	0	81.67 ± 6.38^aA	65.17 ± 7.19^bB	69.25 ± 6.75^bB	52.50 ± 11.95^bC
	0+	86.33 ± 5.42^aA	83.17 ± 4.32^aAB	77.67 ± 6.05^abC	74.67 ± 5.67^aC
	SAE20	82.17 ± 10.63^aA	80.17 ± 5.99^aAB	73.92 ± 3.84^abBC	70.25 ± 6.62^aC
	SAE40	82.00 ± 3.85^aA	80.00 ± 7.87^aAB	73.67 ± 6.19^abB	70.33 ± 6.31^aC
	SAE80	81.50 ± 7.23^aA	79.83 ± 4.36^aA	71.92 ± 3.09^abB	69.58 ± 5.62^aB
I-PLM (%)	0	81.00 ± 5.55	76.17 ± 9.79	75.75 ± 3.49	69.83 ± 6.40
	0+	85.08 ± 5.28	83.67 ± 4.55	81.00 ± 2.89	79.50 ± 6.03
	SAE20	82.67 ± 5.57	81.92 ± 2.87	80.08 ± 4.08	76.33 ± 3.61
	SAE40	83.17 ± 4.62	81.00 ± 13.07	79.50 ± 3.82	75.50 ± 2.51
	SAE80	84.50 ± 6.25	80.33 ± 4.68	80.33 ± 2.96	78.67 ± 4.93
I-DNA (%)	0	99.75 ± 0.27	99.75 ± 0.27	98.50 ± 1.76	94.58 ± 7.93
	0+	99.33 ± 0.75	99.42 ± 0.58	99.25 ± 1.17	99.00 ± 1.14
	SAE20	98.75 ± 1.04	98.42 ± 1.86	98.33 ± 2.96	99.75 ± 0.42
	SAE40	97.67 ± 3.67	97.75 ± 3.73	97.50 ± 3.58	99.17 ± 0.82
	SAE80	98.33 ± 1.25	99.83 ± 0.26	99.58 ± 0.58	99.58 ± 0.20

h, hour; %, percentage; 0, neutral control group with 0 μg/mL of SAE; 0+, positive control group with 0 μg/mL of SAE plus 0.3 g/dL of vitamin C, 100 IU/mL of penicillin and 0.05 mg/mL of streptomycin; SAE20, SAE40, and SAE80 groups with 20, 40, and 80 μg of Soursop Aqueous extract per mL of extender; I-PLM, Intact plasma membrane; I-DNA, Intact DNA. ^a,bValues with different superscripts indicate significant differences (p < 0.05) among treatments at each time point. ^A,B,CValues with different superscripts indicate significant differences (p < 0.05) among storage time points for each treatment.

Journal of Animal Reproduction and Biotechnology 2024;39:179-93 https://doi.org/10.12750/JARB.39.3.179