Journal of Animal Reproduction and Biotechnology

Table. 8.

Table 8

Simple interactions of time and doses of Annona muricata aqueous extract on oxidative stress biomarkers of spermatozoa in buck semen extended in Tris-egg yolk extender and stored at 4℃

	POX	MDA	CAT	SOD
Doses
0	27.29 ± 6.46^b	2.02 ± 0.67^a	12.59 ± 2.70	1.68 ± 0.53^b
0+	34.12 ± 8.16^a	1.34 ± 0.36^b	15.17 ± 2.77	2.22 ± 0.49^a
SAE20	30.51 ± 6.12^ab	1.55 ± 0.52^b	13.87 ± 5.43	1.95 ± 0.64^ab
SAE40	30.25 ± 5.88^ab	1.53 ± 0.35^b	14.51 ± 3.48	1.79 ± 0.60^b
SAE80	30.49 ± 7.29^ab	1.41 ± 0.36^b	15.09 ± 5.12	1.89 ± 0.53^b
Times
6 h	33.87 ± 7.24^a	1.40 ± 0.49	15.73 ± 3.99^a	2.18 ± 0.53^a
24 h	31.18 ± 6.90^ab	1.59 ± 0.49	14.85 ± 4.21^ab	2.06 ± 0.55^a
48 h	28.87 ± 6.64^b	1.59 ± 0.47	13.47 ± 4.34^b	1.78 ± 0.54^b
72 h	28.21 ± 6.32^b	1.70 ± 0.59	12.94 ± 3.45^b	1.61 ± 0.56^b
p-value
Doses	^*	^***	^•	^**
Times	^**	^*	^*	^***
Doses × times	^-	^-	^-	^-

h, hour; %, percentage; 0, neutral control group with 0 μg/mL of SAE; 0+, positive control group with 0 μg/mL of SAE plus 0.3 g/dL of vitamin C, 100 IU/mL of penicillin and 0.05 mg/mL of streptomycin; SAE20, SAE40, and SAE80 groups with 20, 40, and 80 μg of Soursop Aqueous extract per mL of extender; POX, peroxidase; MDA, malondialdehyde; CAT, catalase; SOD, superoxide dismutase; ^a,bValues with different superscripts indicate significant differences (p < 0.05) among storage time points for each treatment or significant differences (p < 0.05) among treatments at each time point; ***(p ≤ 0.000) = strongly significant; **(p ≤ 0.001) = moderately significant; *(p ≤ 0.05) = weakly significant; -(p ≥ 1) = strongly insignificant; •(p ≥ 0.1) = weakly insignificant.

Journal of Animal Reproduction and Biotechnology 2024;39:179-93 https://doi.org/10.12750/JARB.39.3.179