Histological abnormality and fibrosis after inducing renal injury. Histological comparison between the sham group and 2 weeks treatment of 10 mg/kg of cisplatin with LPS 5 mg/kg group (Cis 10 + 2-wk) were performed using H&E (A) and MT (B) staining. Sham group received saline injections. Compared to the sham group, notable renal injury features, including tubule dilation (black arrow), cast formation (red arrow), and brush-border loss (blue arrow), were observed, along with evidence of fibrosis. Sham group received saline injections. H&E, hematoxylin and eosin staining; MT, Masson’s trichrome staining; Cis, cisplatin; Cis 10 + 2-wk, cisplatin 10 mg/kg + LPS 5 mg/kg treatment for 2 weeks. Scale bar represents 50 μm.