Transduction efficiency of AAV serotypes containing eGFP on day 3 post-infection in pLCsImt. (A) Representative eGFP (green) fluorescence images in pLCsImt three days after transduction of various AAV vectors (AAV1–9, 6.2, rh10, DJ, DJ/8, PHP.eB, PHP.S, 2-retro, 2-QuadYF, and 2.7m8). Individual AAV serotypes were infected at MOIs of 20 K for 18 h. The mock was not treated with the AAV vector and served as the negative control. Nuclei were stained with Hoechst 33342 (blue). Scale bars indicate 250 μm. (B) Quantification of eGFP-positive cells in different AAV serotype-infected pLCsImt. Data are presented as a mean ± standard error. Two independent co-workers counted. Different letters denote significant differences within each group, a-dp < 0.05 (n = 3). (C) Comparison with the mRNA expression level of eGFP in AAV serotype infected-pLCsImt. Relative quantification (RQ) of the gene expression was calculated using the ΔΔCt method, with data presented as RQ. Error bars represent the RQ of the minimum and maximum expression levels around the mean. All of quantification were normalized to the internal housekeeping gene GAPDH. Different letters denote significant differences within each group, a-kp < 0.05. All experiments were performed in triplicate. AAV, adeno-associated virus; eGFP, enhanced green fluorescence protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MOI, multiplicity of infection; pLCsImt, immortalized porcine lung epithelial cells; RQ, relative quantification.