Transduction efficiency of individual AAV serotypes containing eGFP on day 3 post-infection in PAMs 3D4/31. (A) Representative eGFP (green) fluorescence images in PAMs 3D4/31 three days after transduction of various AAV vectors. Individual AAV serotypes were infected at MOIs of 20 K for 18 h. The mock was not treated with the AAV vector and served as the negative control. Nuclei were stained with Hoechst 33342 (blue). Scale bars indicate 250 μm. (B) Quantification of eGFP-positive cells in different AAV serotype-infected PAMs 3D4/31. Data were presented as a mean ± standard error. Two independent co-workers counted. Different letters denote significant differences within each group, a-fp < 0.05 (n = 3). (C) Comparison with the mRNA expression level of eGFP in AAV serotype infected-PAMs 3D4/31. RQ of the gene expression was calculated using the ΔΔCt method, with data presented as RQ. Error bars represent the RQ for the minimum and maximum expression levels around the mean. All of quantification were normalized to the internal housekeeping gene GAPDH. Different letters denote significant differences within each group, a-ip < 0.05. All experiments were performed in triplicate. PAMs 3D4/31, pulmonary alveolar macrophages 3D4/31.