Expression of vascular endothelial cells marker (CD-31) protein in cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs. (A) Immunostaining images of vascular endothelial cells marker (CD-31) protein in porcine epiblast stem cells (pEpiSCs), porcine bone marrow-derived mesenchymal stem cells (pBM-MSCs) and porcine adipose-derived mesenchymal stem cells (pA-MSCs) cultured in differentiation medium (EBM-2 + 50 ng/mL VEGF) on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Green: staining of CD-31 protein. Blue: staining of Hoechst 33342, Merge: CD-31 protein and Hoechst 33342 signals. Scale bar = 25 µm. (B) Relative mRNA levels of CD-31 in differentiated cells from pEpiSCs, pBM-MSCs and pA-MSCs cultured in EBM-2 + 50 ng/mL VEGF on cultured plates coated with matrigel® for 8 days. Values presented as mean SEM. *p < 0.05, **p < 0.01 versus SUVEC. SUVEC were used as the control. (C) Flow cytometry of CD-31 expression in unsorted and sorted cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs, respectively.