Expression of pluripotency marker (OCT-3/4) protein in cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs. (A) Immunocytochemistry of pluripotency marker (OCT-3/4) protein in porcine epiblast stem cells (pEpiSCs), porcine bone marrow-derived mesenchymal stem cells (pBM-MSCs) and porcine adipose-derived mesenchymal stem cells (pA-MSCs), respectively. Green: staining of OCT-3/4 protein. Blue: staining of Hoechst 33342, Merge: OCT-3/4 protein and Hoechst 33342 signals. (B) Immunocytochemistry of pluripotency marker (OCT-3/4) protein in cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs cultured in EBM-2 + 50 ng/mL VEGF on cultured plates coated with matrigel® for 8 days, respectively. Red: staining of OCT-3/4 protein. Blue: staining of Hoechst 33342, Merge: OCT-3/4 protein and Hoechst 33342 signals. Scale bar = 100 µm.