Three-dimensional spheroids sprouting of CD-31 positive (+) sorted cells differentiated from pEpiSCs, pBM-MSCs and pA-MSCs. (A) Three-dimensional (3D) spheroids derived from CD-31 positive (+) sorted cells differentiated from porcine epiblast stem cells (pEpiSCs), porcine bone marrow-derived mesenchymal stem cells (pBM-MSCs) and porcine adipose-derived mesenchymal stem cells (pA-MSCs) using the hanging drop protocol in collagen type I mixtures supplemented with ECGS for 24 hours. Scale bar = 100 µm. (B) Sprouts length was calculated by using Image J. *,**Values in same columns with different superscripts are significantly different (p < 0.05).