JARB Journal of Animal Reproduction and Biotehnology

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Journal of Embryo Transfer 1996; 11(3): 233-240

Published online December 1, 1996

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

돼지 未成熟 卵胞卵의 琉璃化 凍結融解後 FDA 처리가 體外受精과 胚 凍結에 미치는 影響 Ⅱ. 卵丘細胞의 附着程度가 돼지난포란의 體外成熟에 미치는 影響

金瑩勳, 金重桂

濟州大學校 農科大學 畜産學科

Abstract

This study was carried out to determine the effect of cumulus cell attachment and various factors on in vitro maturation of pig foflicular oocytes. Oocytes with various configuration of cumulus cell mass were collected ftom ovaries of mature gilts by asperating with syringe equipped with needles of different gauges, follicle size and with or without cumulus cells. They were cultured in TCM-199 mediun containing FGS(fetal calf serum) for 30~48 hours in incubator with air containing 5% $CO_2$ at 38.5$^{circ}C$. Mter orcein staining at in vitro maturation condition, GV, GVBD, anaphase, telophase and M II were observed. Results are surumarized as follows: 1. Recovery rates were 55.8, 55.5 and 34.4% when the cumulus-compacted oocytes were collected with 18, 21, 26 gauge needles of syringes, respectively. 2. 79% of oocytes with compacted cumulus cells were at GV stage and most of the oocytes with partially denuded and denuded cumulus cells were from GVBD to M- II stages. 3. Percentage of mature oocytes among those which are follicular diameter of 1~2, 3~6 and over 6 mm was 42.6, 53.2 and 60.8%, respectively. 4. Percentage of mature oocytes among those which are compacted, partially denuded and denuded was 60.5, 46.2 and 35.4% respectively. 5. Percentage of mature oocytes in co-cultured with monolayers of cumulus cells was higher (57.1%) than that found with oocytes cultured alone (53.4%).

Keywords: In vitro fertilization, maturation, porcine, oocytes, cumulus cell, GV stage, GVBD, compacted

Article

Journal of Embryo Transfer 1996; 11(3): 233-240

Published online December 1, 1996

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

돼지 미성숙 난포란의 유리화 동결융해후 FDA 처리가 체외수정과 배 발육에 미치는 영향 II. 난구세포의 부착정도가 돼지난포란의 체외성숙에 미치는 영향

김영훈, 김중계

제주대학교 농과대학, 제주대학교 농과대학

돼지 未成熟 卵胞卵의 琉璃化 凍結融解後 FDA 처리가 體外受精과 胚 凍結에 미치는 影響 Ⅱ. 卵丘細胞의 附着程度가 돼지난포란의 體外成熟에 미치는 影響

金瑩勳, 金重桂

濟州大學校 農科大學 畜産學科

Abstract

This study was carried out to determine the effect of cumulus cell attachment and various factors on in vitro maturation of pig foflicular oocytes. Oocytes with various configuration of cumulus cell mass were collected ftom ovaries of mature gilts by asperating with syringe equipped with needles of different gauges, follicle size and with or without cumulus cells. They were cultured in TCM-199 mediun containing FGS(fetal calf serum) for 30~48 hours in incubator with air containing 5% $CO_2$ at 38.5$^{circ}C$. Mter orcein staining at in vitro maturation condition, GV, GVBD, anaphase, telophase and M II were observed. Results are surumarized as follows: 1. Recovery rates were 55.8, 55.5 and 34.4% when the cumulus-compacted oocytes were collected with 18, 21, 26 gauge needles of syringes, respectively. 2. 79% of oocytes with compacted cumulus cells were at GV stage and most of the oocytes with partially denuded and denuded cumulus cells were from GVBD to M- II stages. 3. Percentage of mature oocytes among those which are follicular diameter of 1~2, 3~6 and over 6 mm was 42.6, 53.2 and 60.8%, respectively. 4. Percentage of mature oocytes among those which are compacted, partially denuded and denuded was 60.5, 46.2 and 35.4% respectively. 5. Percentage of mature oocytes in co-cultured with monolayers of cumulus cells was higher (57.1%) than that found with oocytes cultured alone (53.4%).

Keywords: In vitro fertilization, maturation, porcine, oocytes, cumulus cell, GV stage, GVBD, compacted

JARB Journal of Animal Reproduction and Biotehnology

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OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663