JARB Journal of Animal Reproduction and Biotehnology

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eISSN: 2671-4663

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Journal of Embryo Transfer 2003; 18(2): 151-156

Published online September 8, 2003

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

한우의 생식세포 보존에 관한 연구 II. PEG 30% FSH 투여가 한우의 체내수정란 생산에 미치는 영향

이명식, 박정준, 전기준, 정영훈, 우제석, 박수봉, 임석기, 연성흠, 손동수

농촌진흥청 축산기술연구소

Abstract

The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.

Keywords: superovulation, corpus luteum, ovary, transferable embryos, PEG 30% FSH

Article

Journal of Embryo Transfer 2003; 18(2): 151-156

Published online September 8, 2003

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

한우의 생식세포 보존에 관한 연구 II. PEG 30% FSH 투여가 한우의 체내수정란 생산에 미치는 영향

이명식, 박정준, 전기준, 정영훈, 우제석, 박수봉, 임석기, 연성흠, 손동수

농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소, 농촌진흥청 축산기술연구소

한우의 생식세포 보존에 관한 연구 II. PEG 30% FSH 투여가 한우의 체내수정란 생산에 미치는 영향

이명식, 박정준, 전기준, 정영훈, 우제석, 박수봉, 임석기, 연성흠, 손동수

농촌진흥청 축산기술연구소

Abstract

The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.

Keywords: superovulation, corpus luteum, ovary, transferable embryos, PEG 30% FSH