JARB Journal of Animal Reproduction and Biotehnology

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Journal of Embryo Transfer 2005; 20(1): 43-48

Published online April 30, 2005

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

돼지정액의 동결에 관한 연구 Ⅰ.냉각속도와 희석액이 동결융해후 정자의 생존성과 정상첨체율에 미치는 영향

심금섭, 김광식, 서경덕, 송해범

천안연암대학

Abstract

This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was $0.17~022^{circ}C/min$ from 25 to $5^{circ}C$ in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or $4\%$ of glycerol concentration than $2\%$ in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or $4\%$ glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).

Keywords: boar, freeing, extender, glycerol, viability

Article

Journal of Embryo Transfer 2005; 20(1): 43-48

Published online April 30, 2005

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

돼지정액의 동결에 관한 연구 I. 냉각속도와 희석액이 동결융해후 정자의 생존성과 정상첨체율에 미치는 영향

심금섭, 김광식, 서경덕, 송해범

천안연암대학, 천안연암대학, 천안연암대학, 대구대학교

돼지정액의 동결에 관한 연구 Ⅰ.냉각속도와 희석액이 동결융해후 정자의 생존성과 정상첨체율에 미치는 영향

심금섭, 김광식, 서경덕, 송해범

천안연암대학

Abstract

This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was $0.17~022^{circ}C/min$ from 25 to $5^{circ}C$ in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or $4\%$ of glycerol concentration than $2\%$ in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or $4\%$ glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).

Keywords: boar, freeing, extender, glycerol, viability

JARB Journal of Animal Reproduction and Biotehnology

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OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663