JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Journal of Embryo Transfer 2006; 21(2): 163-168

Published online June 30, 2006

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

난소 수송 온도에 따른 소 체외 수정란의 발육 및 동결-융해 후의 생존성

조상래, 최선호, 김현종, 최창용, 진현주, 손동수

농촌진흥청 축산연구소 가축유전자원시험장

Abstract

The present study was carried out to investigate in vitro development and post-thawed survivability of bovine embryos according to different ovary transport temperatures. Bovine ovaries were collected at a local slaughterhouse and were transported at 4 different temperature categories to laboratory: $7{sim}10^{circ}C;(T1),;11{sim}17^{circ}C;(T2),;18{sim}25^{circ}C;(T3)$ and above $26^{circ}C$ (control group). The cumulus-oocyte-complexes aspirated from ovaries were in vitro matured, fertilized and cultured. The rates of maturation (to metaphase II), cleavage and development to blastocysts were compared among treatment groups. Furthermore, frozen-thawed blastocysts were in vitro cultured to compare the survivability among groups. The maturation rates in the T1, T2 and T3 groups ($60.0{sim}68.2%$) were significantly lower than that in the control group (81.8%, p<0.05). The cleavage rates in the T1 and T2 groups (52.6 and 54.5%) were significantly lower than that in the control group (83.6%, p<0.05). However, there was no difference in the development rate to blastocysts among all groups ($27.9{sim}33.0%$, p>0.05). The survivability of frozen-thawed embryos was significantly lower in the T1 group (46.2%) than those in the T2, T3 and control groups ($68.8{sim}7.13%$, p<0.05). In conclusion, the results suggest that ovary transport temperature at $26^{circ}C$ may be optimal for the better in vitro development and the survival of frozen-thawed embryos produced in vitro Furthermore, exposure of ovary to temperature below $10^{circ}C$ during transport may significantly decrease both in vitro development and survivability of frozen-thawed blastocysts.

Keywords: bovine, embryo, ovary, transport, temperature, survivability

Article

Journal of Embryo Transfer 2006; 21(2): 163-168

Published online June 30, 2006

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

난소 수송 온도에 따른 소 체외 수정란의 발육 및 동결-융해 후의 생존성

조상래, 최선호, 김현종, 최창용, 진현주, 손동수

농촌진흥청 축산연구소 가축유전자원시험장, 농촌진흥청 축산연구소 가축유전자원시험장, 농촌진흥청 축산연구소 가축유전자원시험장, 농촌진흥청 축산연구소 가축유전자원시험장, 농촌진흥청 축산연구소 가축유전자원시험장, 농촌진흥청 축산연구소 가축유전자원시험장

난소 수송 온도에 따른 소 체외 수정란의 발육 및 동결-융해 후의 생존성

조상래, 최선호, 김현종, 최창용, 진현주, 손동수

농촌진흥청 축산연구소 가축유전자원시험장

Abstract

The present study was carried out to investigate in vitro development and post-thawed survivability of bovine embryos according to different ovary transport temperatures. Bovine ovaries were collected at a local slaughterhouse and were transported at 4 different temperature categories to laboratory: $7{sim}10^{circ}C;(T1),;11{sim}17^{circ}C;(T2),;18{sim}25^{circ}C;(T3)$ and above $26^{circ}C$ (control group). The cumulus-oocyte-complexes aspirated from ovaries were in vitro matured, fertilized and cultured. The rates of maturation (to metaphase II), cleavage and development to blastocysts were compared among treatment groups. Furthermore, frozen-thawed blastocysts were in vitro cultured to compare the survivability among groups. The maturation rates in the T1, T2 and T3 groups ($60.0{sim}68.2%$) were significantly lower than that in the control group (81.8%, p<0.05). The cleavage rates in the T1 and T2 groups (52.6 and 54.5%) were significantly lower than that in the control group (83.6%, p<0.05). However, there was no difference in the development rate to blastocysts among all groups ($27.9{sim}33.0%$, p>0.05). The survivability of frozen-thawed embryos was significantly lower in the T1 group (46.2%) than those in the T2, T3 and control groups ($68.8{sim}7.13%$, p<0.05). In conclusion, the results suggest that ovary transport temperature at $26^{circ}C$ may be optimal for the better in vitro development and the survival of frozen-thawed embryos produced in vitro Furthermore, exposure of ovary to temperature below $10^{circ}C$ during transport may significantly decrease both in vitro development and survivability of frozen-thawed blastocysts.

Keywords: bovine, embryo, ovary, transport, temperature, survivability