JARB Journal of Animal Reproduction and Biotehnology

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eISSN: 2671-4663

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Journal of Embryo Transfer 2007; 22(3): 155-160

Published online September 29, 2007

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

배양액 및 산소농도가 돼지 체외수정란의 발달에 미치는 영향

최창용, 조상래, 최선호, 김현종, 한만희, 강다원, 신용원, 한재희, 손동수

농촌진흥청 축산과학원 가축유전자원시험장

Abstract

During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen ($O_2$), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% $O_2$, in NCSU-23), the mean cell number per blastocyst was $40{pm}10$. These results showed that medium and $O_2$ concentration had no significant effect on the development of porcine embryos.

Keywords: culture media, oxygen concentration, porcine, blastocyst

Article

Journal of Embryo Transfer 2007; 22(3): 155-160

Published online September 29, 2007

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

배양액 및 산소농도가 돼지 체외수정란의 발달에 미치는 영향

최창용, 조상래, 최선호, 김현종, 한만희, 강다원, 신용원, 한재희, 손동수

농촌진흥청 축산과학원 가축유전자원시험장, 농촌진흥청 축산과학원 가축유전자원시험장, 농촌진흥청 축산과학원 가축유전자원시험장, 농촌진흥청 축산과학원 가축유전자원시험장, 농촌진흥청 축산과학원 가축유전자원시험장, 경상대학교 의과대학 생리학교실, 경상대학교 의과대학 생리학교실, 경상대학교 의과대학 생리학교실, 농촌진흥청 축산과학원 가축유전자원시험장

배양액 및 산소농도가 돼지 체외수정란의 발달에 미치는 영향

최창용, 조상래, 최선호, 김현종, 한만희, 강다원, 신용원, 한재희, 손동수

농촌진흥청 축산과학원 가축유전자원시험장

Abstract

During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen ($O_2$), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% $O_2$, in NCSU-23), the mean cell number per blastocyst was $40{pm}10$. These results showed that medium and $O_2$ concentration had no significant effect on the development of porcine embryos.

Keywords: culture media, oxygen concentration, porcine, blastocyst

JARB Journal of Animal Reproduction and Biotehnology

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OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663