JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

Article Search

Article
Split Viewer

Journal of Embryo Transfer 2008; 23(3): 183-187

Published online September 30, 2008

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

랫드 난소에서 난포 발달에 따른 DNA 결합 단백질 억제인자 (Inhibitor of DNA Binding Protein) Id1과 Id2 mRNA 발현

황성수, 김평희, 고응규, 양병철, 성환후, 민관식, 윤종택

농촌진흥청 축산과학원 응용생명공학과

Abstract

This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.

Keywords: inhibitor of DNA binding protein (Id)l and Id2, ovary, folliculogenesis, rat, in situ hybridization

Article

Journal of Embryo Transfer 2008; 23(3): 183-187

Published online September 30, 2008

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

랫드 난소에서 난포 발달에 따른 DNA 결합 단백질 억제인자 (Inhibitor of DNA Binding Protein) Id1 and Id2 mRNA 발현

황성수, 김평희, 고응규, 양병철, 성환후, 민관식, 윤종택

농촌진흥청 축산과학원 응용생명공학과, 농촌진흥청 축산과학원 응용생명공학과, 농촌진흥청 축산과학원 응용생명공학과, 농촌진흥청 축산과학원 응용생명공학과, 농촌진흥청 축산과학원 응용생명공학과, 한경대학교 생물.환경정보통신전문대학원, 한경대학교 동물자원과학과

랫드 난소에서 난포 발달에 따른 DNA 결합 단백질 억제인자 (Inhibitor of DNA Binding Protein) Id1과 Id2 mRNA 발현

황성수, 김평희, 고응규, 양병철, 성환후, 민관식, 윤종택

농촌진흥청 축산과학원 응용생명공학과

Abstract

This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.

Keywords: inhibitor of DNA binding protein (Id)l and Id2, ovary, folliculogenesis, rat, in situ hybridization