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Journal of Embryo Transfer 2010; 25(1): 15-20

Published online March 31, 2010

## OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향

진종인, 권태현, 최병현, 김성수, 조현태, 공일근

경상대학교 응용생명과학부 축산학전공(BK21)

### Abstract

This study was performed to identify the optimal timing for oocyte donor replacement during OPU procedure. OPU was carried out to collect oocytes from every donor at an interval of $3{sim}4$ days (2 times a week). The collected oocytes were matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml of FSH and 1 mg/ml of estradiol for 24 h. After 24 h of exposure to sperm, the presumptive zygotes were cultured in CR1aa medium supplemented with 4 mg/ml of BSA for 3 days before being changed to CR1aa medium with 10% of FBS for another $3{sim}4$ days. The mean numbers of retrieved oocytes were remained constantly up to 3 months ($6.0{pm}0.5$, $6.2{pm}0.7$, $5.2{pm}0.6$), but significantly decreased at over 4 to 6 months ($3.7{pm}0.5$, $2.8{pm}0.4$, $1.2{pm}0.2$) (p<0.05). The blastocyst development potential was also very similar rate from 1 to 3 months (37.2%, 40.4% and 44.6%), but significantly decreased from 4 to 6 months (24.8%, 29.3% and 28.6%, respectively) (p<0.05). The production of OPU derived embryos in periods of 1 to 3 months ($2.2{pm}0.3$, $2.5{pm}0.3$ and $2.3{pm}0.4$) were significantly higher than those in 4 to 6 months ($0.9{pm}0.2$, $0.8{pm}0.2$ and $0.3{pm}0.2$, respectively) (p<0.05). In conclusion, the efficient periods for the production of OPU derived embryos was until 4 months, twice per week to produce over 64 transferable embryos and then replace new donor after 3 months use. The best replacement time is 3 months and could be maximized production of OPU derived embryos.

Keywords: OPU, duration, bovine, oocyte, blastocyst

### Article

Journal of Embryo Transfer 2010; 25(1): 15-20

Published online March 31, 2010

## OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향

진종인, 권태현, 최병현, 김성수, 조현태, 공일근

경상대학교 응용생명과학부 축산학(BK21), 경상대학교 응용생명과학부 축산학(BK21), 경상대학교 응용생명과학부 축산학(BK21), 경상대학교 응용생명과학부 축산학(BK21), 경상대학교 응용생명과학부 축산학(BK21), 경상대학교 응용생명과학부 축산학(BK21)

## OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향

진종인, 권태현, 최병현, 김성수, 조현태, 공일근

경상대학교 응용생명과학부 축산학전공(BK21)

### Abstract

This study was performed to identify the optimal timing for oocyte donor replacement during OPU procedure. OPU was carried out to collect oocytes from every donor at an interval of $3{sim}4$ days (2 times a week). The collected oocytes were matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml of FSH and 1 mg/ml of estradiol for 24 h. After 24 h of exposure to sperm, the presumptive zygotes were cultured in CR1aa medium supplemented with 4 mg/ml of BSA for 3 days before being changed to CR1aa medium with 10% of FBS for another $3{sim}4$ days. The mean numbers of retrieved oocytes were remained constantly up to 3 months ($6.0{pm}0.5$, $6.2{pm}0.7$, $5.2{pm}0.6$), but significantly decreased at over 4 to 6 months ($3.7{pm}0.5$, $2.8{pm}0.4$, $1.2{pm}0.2$) (p<0.05). The blastocyst development potential was also very similar rate from 1 to 3 months (37.2%, 40.4% and 44.6%), but significantly decreased from 4 to 6 months (24.8%, 29.3% and 28.6%, respectively) (p<0.05). The production of OPU derived embryos in periods of 1 to 3 months ($2.2{pm}0.3$, $2.5{pm}0.3$ and $2.3{pm}0.4$) were significantly higher than those in 4 to 6 months ($0.9{pm}0.2$, $0.8{pm}0.2$ and $0.3{pm}0.2$, respectively) (p<0.05). In conclusion, the efficient periods for the production of OPU derived embryos was until 4 months, twice per week to produce over 64 transferable embryos and then replace new donor after 3 months use. The best replacement time is 3 months and could be maximized production of OPU derived embryos.

Keywords: OPU, duration, bovine, oocyte, blastocyst

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