JARB Journal of Animal Reproduction and Biotehnology

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Journal of Embryo Transfer 2010; 25(3): 195-199

Published online September 30, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

진돗개 정액 동결 시 단당류 및 동결 속도가 생존율에 미치는 영향

최선호, 김성재, 조상래, 최창용, 손준규, 김종석, 오석일, 박병진, 김상현

농촌진흥청 국립축산과학원

Abstract

This studies were conducted to investigate the survival rate of frozen-thawed spermatozoa of Jindo Dog by monosaccharide and freezing rates. Experimental animals were prepared 12 males within 1~8 year's old and collected once in a couple of weeks by digital manuplation methods. Collected semen was diluted 1:1 with Tris-egg yolk extender and added 4, 6 or 8% of glycerol and none, 4 mM glucose or 4 mM fructose as cryoprotectant and was equilibrated for 2 hrs in $4^{circ}C$. In monosaccharide groups, the freezing rate was 5 cm-5 min. above $LN_2$. The survival rates without monosaccharide were $50.7{pm}19.0%$, $58.6{pm}18.0%$, $40.0{pm}10.0%$ in 4, 6 or 8% glycerol, respectively. In addition of glucose, the survival rates were $43.1{pm}14.7%$, $38.1{pm}16.5%$, $33.3{pm}4.0%$ in 4, 6 or 8% glycerol, respectively and in fructose, were $47.9{pm}21.1%$, $61.3{pm}6.2%$, $34.3{pm}12.6%$ in 4, 6 or 8% glycerol, respectively. There showed significantly different between glycerol groups and monosaccharides groups (p<0.05). The survival rates of freezing rate in 5 cm-5 min. group was $64.5{pm}15.8%$, $51.9{pm}27.6%$, $29.7{pm}24.8%$ and in 10 cm-10 min. group was $62.5{pm}20.3%$, $64.9{pm}23.6%$, $34.5{pm}27.4%$ in 4, 6 or 8% glycerol, respectively. There were significantly different between freezing rates (p<0.05). These results suggest that the addition of fructose with 6%-glycerol and slow freezing improve the survival of frozen-thawed sperm in Jindo Dog.

Keywords: Jindo Dog, spermatozoa, freezing, monsaccharides, survival

Article

Journal of Embryo Transfer 2010; 25(3): 195-199

Published online September 30, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

진도개 정액 동결 시 단당류 및 동결 속도가 생존율에 미치는 영향

최선호, 김성재, 조상래, 최창용, 손준규, 김종석, 오석일, 박병진, 김상현

농촌진흥청 국립축산과학원, 농촌진흥청 국립축산과학원, 농촌진흥청 국립축산과학원, 농촌진흥청 국립축산과학원, 농촌진흥청 국립축산과학원, 진돗개사업소, 진돗개사업소, 진돗개사업소, 진도개사업소

진돗개 정액 동결 시 단당류 및 동결 속도가 생존율에 미치는 영향

최선호, 김성재, 조상래, 최창용, 손준규, 김종석, 오석일, 박병진, 김상현

농촌진흥청 국립축산과학원

Abstract

This studies were conducted to investigate the survival rate of frozen-thawed spermatozoa of Jindo Dog by monosaccharide and freezing rates. Experimental animals were prepared 12 males within 1~8 year's old and collected once in a couple of weeks by digital manuplation methods. Collected semen was diluted 1:1 with Tris-egg yolk extender and added 4, 6 or 8% of glycerol and none, 4 mM glucose or 4 mM fructose as cryoprotectant and was equilibrated for 2 hrs in $4^{circ}C$. In monosaccharide groups, the freezing rate was 5 cm-5 min. above $LN_2$. The survival rates without monosaccharide were $50.7{pm}19.0%$, $58.6{pm}18.0%$, $40.0{pm}10.0%$ in 4, 6 or 8% glycerol, respectively. In addition of glucose, the survival rates were $43.1{pm}14.7%$, $38.1{pm}16.5%$, $33.3{pm}4.0%$ in 4, 6 or 8% glycerol, respectively and in fructose, were $47.9{pm}21.1%$, $61.3{pm}6.2%$, $34.3{pm}12.6%$ in 4, 6 or 8% glycerol, respectively. There showed significantly different between glycerol groups and monosaccharides groups (p<0.05). The survival rates of freezing rate in 5 cm-5 min. group was $64.5{pm}15.8%$, $51.9{pm}27.6%$, $29.7{pm}24.8%$ and in 10 cm-10 min. group was $62.5{pm}20.3%$, $64.9{pm}23.6%$, $34.5{pm}27.4%$ in 4, 6 or 8% glycerol, respectively. There were significantly different between freezing rates (p<0.05). These results suggest that the addition of fructose with 6%-glycerol and slow freezing improve the survival of frozen-thawed sperm in Jindo Dog.

Keywords: Jindo Dog, spermatozoa, freezing, monsaccharides, survival

JARB Journal of Animal Reproduction and Biotehnology

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OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663