JARB Journal of Animal Reproduction and Biotehnology

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Journal of Embryo Transfer 2011; 26(2): 129-133

Published online June 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Genistein Prevents Ethanol-Induced Teratogenesis in Mouse Embryos

Yon, Jung-Min, Lin, Chunmei, Jung, A-Young, Lee, Jong-Geol, Jung, Ki-Youn, Baek, In-Jeoung, Lee, Beom-Jun, Yun, Young-Won, Nam, Sang-Yoon

Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Ceongju 361-763, Korea

Abstract

Drinking of excessive ethanol during pregnancy induces a fetal alcohol syndrome. Genistein is one of naturally occurring isoflavones at relatively high levels in soybeans. In this study, we investigated the effects of genistein ($1{ imes}10^{-8}$ and $1{ imes}10^{-7};{mu}g$/ml) on the ethanol (1 ${mu}l$/ml)-induced teratogenesis of developing mouse embryos during the critical period (embryonic days 8.5~10.5) of organogenesis using a whole embryo culture system and then morphological scoring analysis. Ethanol-treated embryos exhibited a variety of developmental abnormalities. However, the total morphological scores for ethanol plus genistein groups were significantly higher than those of ethanol alone group (p<0.05). In particular, there were significant increases in the ethanol plus $1{ imes}10^{-8};{mu}g$/ml of genistein group on the scores for heart, optic system, branchial bar, mandibular process, and caudal neural tube and further in the ethanol plus $1{ imes}10^{-7};{mu}g$/ml of genistein group on the scores for heart, hind-, mid-, and forebrains, optic system, branchial bars, maxillary and mandibular processes, caudal neural tube, forelimb, hindlimb, and somites as compared with those of ethanol alone group (p<0.05). These results indicate that genistein has a preventive effect against ethanol-induced teratogenesis.

Keywords: ethanol, genistein, teratogenesis, whole embryo culture

Article

Journal of Embryo Transfer 2011; 26(2): 129-133

Published online June 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Genistein Prevents Ethanol-Induced Teratogenesis in Mouse Embryos

Yon, Jung-Min, Lin, Chunmei, Jung, A-Young, Lee, Jong-Geol, Jung, Ki-Youn, Baek, In-Jeoung, Lee, Beom-Jun, Yun, Young-Won, Nam, Sang-Yoon

Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Ceongju 361-763, Korea

Abstract

Drinking of excessive ethanol during pregnancy induces a fetal alcohol syndrome. Genistein is one of naturally occurring isoflavones at relatively high levels in soybeans. In this study, we investigated the effects of genistein ($1{ imes}10^{-8}$ and $1{ imes}10^{-7};{mu}g$/ml) on the ethanol (1 ${mu}l$/ml)-induced teratogenesis of developing mouse embryos during the critical period (embryonic days 8.5~10.5) of organogenesis using a whole embryo culture system and then morphological scoring analysis. Ethanol-treated embryos exhibited a variety of developmental abnormalities. However, the total morphological scores for ethanol plus genistein groups were significantly higher than those of ethanol alone group (p<0.05). In particular, there were significant increases in the ethanol plus $1{ imes}10^{-8};{mu}g$/ml of genistein group on the scores for heart, optic system, branchial bar, mandibular process, and caudal neural tube and further in the ethanol plus $1{ imes}10^{-7};{mu}g$/ml of genistein group on the scores for heart, hind-, mid-, and forebrains, optic system, branchial bars, maxillary and mandibular processes, caudal neural tube, forelimb, hindlimb, and somites as compared with those of ethanol alone group (p<0.05). These results indicate that genistein has a preventive effect against ethanol-induced teratogenesis.

Keywords: ethanol, genistein, teratogenesis, whole embryo culture

JARB Journal of Animal Reproduction and Biotehnology

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OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663