JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Journal of Embryo Transfer 1999; 14(2): 131-137

Published online August 1, 1999

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과

정영호, 서경덕, 김광식, 심금섭, 이장희

중부대학교 생명자원학부

Abstract

This experiment was carried out to investigate the effects of osmolarity of thawing diluents, seminal plasma added in thawing diluents on the sperm viability and the effects of thawing temperature, the temparature of the thawing diluents on the sperm viability and acrosomal morphology of boar spermatozoa by the straw method. The result obtained were summarized as follows: 1. The sperm viablilty after thawing of the frozen semen was shown greater in the high osmolarity(392~492mOsm) than low osmolarity(300mOsm) in thawing diluent. The added levels of seminal plasma in thawing diluent did not affect the viability of frozen-thawed boar semen. 2. In terms of thawing temperature, the sperm viability was shown higher in the frozen semen thawed at 5$0^{circ}C$ for one min. (p<0.01) than those thawed at 2$0^{circ}C$ or 37$^{circ}C$ for one min. The sperm viability was not significant at the diluent temparature of 2$0^{circ}C$or 37$^{circ}C$ after thawing: but the sperm viability was higher in thawing diluent at 2$0^{circ}C$ than in that at 37$^{circ}C$. However, the effects of thawing temperature and diluent solution on normal acrosomal rate were not significant. 3. Cleavage rates of oocytes fertilized with frozen semen were 46.4% and 43.3%, respectively, which were thawed at 5$0^{circ}C$ for one min. and then diluted in mBTS medium at 2$0^{circ}C$or 37$^{circ}C$. To sum up, the sperm viability was shown greater at the high of thawing diluents of frozen boar semen. In terms of thawing conditions, the sperm viability was shown greater, when semen was thawed at a high temperature for a short time and then diluted at the same temperature as that in the straw.

Keywords: boar, cryopreservation, thawing diluents, viability, acrosome morphology

Article

Journal of Embryo Transfer 1999; 14(2): 131-137

Published online August 1, 1999

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과

정영호, 서경덕, 김광식, 심금섭, 이장희

중부대하? 생명자원학부, 연암축산원예대학, 연암축산원예대학, 연암축산원예대학, 축산기술연구소

동결보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과

정영호, 서경덕, 김광식, 심금섭, 이장희

중부대학교 생명자원학부

Abstract

This experiment was carried out to investigate the effects of osmolarity of thawing diluents, seminal plasma added in thawing diluents on the sperm viability and the effects of thawing temperature, the temparature of the thawing diluents on the sperm viability and acrosomal morphology of boar spermatozoa by the straw method. The result obtained were summarized as follows: 1. The sperm viablilty after thawing of the frozen semen was shown greater in the high osmolarity(392~492mOsm) than low osmolarity(300mOsm) in thawing diluent. The added levels of seminal plasma in thawing diluent did not affect the viability of frozen-thawed boar semen. 2. In terms of thawing temperature, the sperm viability was shown higher in the frozen semen thawed at 5$0^{circ}C$ for one min. (p<0.01) than those thawed at 2$0^{circ}C$ or 37$^{circ}C$ for one min. The sperm viability was not significant at the diluent temparature of 2$0^{circ}C$or 37$^{circ}C$ after thawing: but the sperm viability was higher in thawing diluent at 2$0^{circ}C$ than in that at 37$^{circ}C$. However, the effects of thawing temperature and diluent solution on normal acrosomal rate were not significant. 3. Cleavage rates of oocytes fertilized with frozen semen were 46.4% and 43.3%, respectively, which were thawed at 5$0^{circ}C$ for one min. and then diluted in mBTS medium at 2$0^{circ}C$or 37$^{circ}C$. To sum up, the sperm viability was shown greater at the high of thawing diluents of frozen boar semen. In terms of thawing conditions, the sperm viability was shown greater, when semen was thawed at a high temperature for a short time and then diluted at the same temperature as that in the straw.

Keywords: boar, cryopreservation, thawing diluents, viability, acrosome morphology