JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Journal of Embryo Transfer 2005; 20(2): 177-184

Published online August 31, 2005

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

한우 인공수정시 rbST 투여가 수태 및 분만율에 미치는 영향

한만희, 최선호, 최연호, 김현종, 조상래, 최창용, 류일선, 손동수, 연성흠, 우제석, 권응기, 윤기영, 장병선

농촌진흥청 축산연구소

Abstract

This study was conducted to investigate the effects of recombinant bovine somatotropin (rbST) injection on conception and parturition rates in normal or repeat breeding Hanwoo. We treated 462 cows containing 79 repeat-breeding cows of multiparous and allocating 5 treatment groups. Treatment 1 (T1) was injection of 2ml saline (for pseudo treatment), T2 was one injection of rbST 250mg into the tailhead region at the estrus, T3 was twice injection of rbST 250mg both at the time of insemination and again 10 to 14 day later, T4 was once injection of rbST 500mg at insemination and T5 was twice injection of 500mg rbST both at the time of insemination and again 10 to 14day later respectively. In rbST treated groups, timed artificial inseminations (TAI) were performed fellowing estrus synchronization. 100 us GnRH was injected into the scapula region on Day 0, 25mg $PGF_2{alpha}$ was injected on Day 7 for degeneration of corpus luteum (CL) and 100ug GnRH was injected for inducing the synchronization. The results are as fellows; When normal Hanwoo were inseminated once with rbST administration, the pregnancy rate of T2 $(67.5pm18.48\%)$ were higher than control $(52.4pm9.72\%)$, while the pregnancy rate of T4 $(63.3pm5.77\%)$ were significantly higher (p.<0.05) than control $(39.3pm12.89\%)$ in repeat breeder Hanwoo. The parturition rates of normal Hanwoo were no differences among the treatments but were significant different in repeat breeder Hanwoo (p<0.05). When the estrous was induced by Ovsynch and inseminated once with rbST administration, the pregnancy rates of T2 was $12.5\%$ higher than control in normal Hanwoo, T4 $(80.0\%)$ was highest among the treatments (p<0.05) in repeat breeder Hanwoo. When normal Hanwoo were inseminated once with rbST administration, the pregnant period was $282.7~284.8$ days and the body weight was $25.1~25.9kg$, there were no difference among the treatments. The ratio of sex was almost same without T4 (male vs. female=18:9). In repeat breeder Hanwoo, pregnant period was 280.4~289.3 day and body weight was $23.0~26.6kg$, it had no difference among the treatments. The sex ratio were similar to normal Hanwoo except T4 (M : F=2 : 8). In conclusion, the pregnancy and parturition rate by once insemination could be improved by the administration of rbST 250mg in normal Hanwoo or 500mg in repeat breeder Hawoo.

Keywords: artificial insemination, conception and paturition rates, Hanwoo

Article

Journal of Embryo Transfer 2005; 20(2): 177-184

Published online August 31, 2005

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

한우 인공수정시 rbST 투여가 수태 및 분만율에 미치는 영향

한만희, 최선호, 최연호, 김현종, 조상래, 최창용, 류일선, 손동수, 연성흠, 우제석, 권응기, 윤기영, 장병선

농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, 농촌진흥청 축산연구소, ㈜LG 생명화학, ㈜LG 생명화학

한우 인공수정시 rbST 투여가 수태 및 분만율에 미치는 영향

한만희, 최선호, 최연호, 김현종, 조상래, 최창용, 류일선, 손동수, 연성흠, 우제석, 권응기, 윤기영, 장병선

농촌진흥청 축산연구소

Abstract

This study was conducted to investigate the effects of recombinant bovine somatotropin (rbST) injection on conception and parturition rates in normal or repeat breeding Hanwoo. We treated 462 cows containing 79 repeat-breeding cows of multiparous and allocating 5 treatment groups. Treatment 1 (T1) was injection of 2ml saline (for pseudo treatment), T2 was one injection of rbST 250mg into the tailhead region at the estrus, T3 was twice injection of rbST 250mg both at the time of insemination and again 10 to 14 day later, T4 was once injection of rbST 500mg at insemination and T5 was twice injection of 500mg rbST both at the time of insemination and again 10 to 14day later respectively. In rbST treated groups, timed artificial inseminations (TAI) were performed fellowing estrus synchronization. 100 us GnRH was injected into the scapula region on Day 0, 25mg $PGF_2{alpha}$ was injected on Day 7 for degeneration of corpus luteum (CL) and 100ug GnRH was injected for inducing the synchronization. The results are as fellows; When normal Hanwoo were inseminated once with rbST administration, the pregnancy rate of T2 $(67.5pm18.48\%)$ were higher than control $(52.4pm9.72\%)$, while the pregnancy rate of T4 $(63.3pm5.77\%)$ were significantly higher (p.<0.05) than control $(39.3pm12.89\%)$ in repeat breeder Hanwoo. The parturition rates of normal Hanwoo were no differences among the treatments but were significant different in repeat breeder Hanwoo (p<0.05). When the estrous was induced by Ovsynch and inseminated once with rbST administration, the pregnancy rates of T2 was $12.5\%$ higher than control in normal Hanwoo, T4 $(80.0\%)$ was highest among the treatments (p<0.05) in repeat breeder Hanwoo. When normal Hanwoo were inseminated once with rbST administration, the pregnant period was $282.7~284.8$ days and the body weight was $25.1~25.9kg$, there were no difference among the treatments. The ratio of sex was almost same without T4 (male vs. female=18:9). In repeat breeder Hanwoo, pregnant period was 280.4~289.3 day and body weight was $23.0~26.6kg$, it had no difference among the treatments. The sex ratio were similar to normal Hanwoo except T4 (M : F=2 : 8). In conclusion, the pregnancy and parturition rate by once insemination could be improved by the administration of rbST 250mg in normal Hanwoo or 500mg in repeat breeder Hawoo.

Keywords: artificial insemination, conception and paturition rates, Hanwoo