JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Journal of Embryo Transfer 2011; 26(3): 209-214

Published online September 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Alteration in Two-pore Domain K$^+$ Channel Expression in Endometrium of Pregnant Korean Cattle

최창용, 탁현민, 김창운, 한재희, 강다원

농촌진흥청 국립축산과학원, 경상대학교 의학전문대학원 생리학교실/건강과학연구원, 성균관대학교 삼성창원병원

Abstract

Endometrium undergoing hormonal change plays important roles in preparation for implantation, fetal growth, and well-being. During pregnancy, cellular remodeling and hormonal changes in endometrium could change two-pore domain K$^+$ channel (K$_{2P}$) expression. This study was performed to identify whether K$_{2P}$ channel expression is changed in endometrium of pregnant Korean cattle, and whether the expression level is modulated by progesterone treatment. We investigated changes in the mRNA and protein expressions of K$_{2P}$ channel in pregnant endometrium using RT-PCR and Western blot analyses. The expression levels of all K$_{2P}$ channel mRNAs tested in this study, except that of TREK-1, were changed in the pregnant endometrium. mRNA levels of TASK-3 and TRAAK were significantly down-regulated, whereas those of TREK-2 and TRESK were up-regulated in the pregnant endometrium. In parallel with the RT-PCR results, Western blot analysis revealed up-regulations of TREK-2 (7.9-fold) and TRESK (2-fold) proteins levels in the pregnant endometrium. In addition, TREK-2 and TRESK protein levels were up-regulated in bovine endometrial cells by progesterone treatment (10 ${mu}g$/ml). From these results, we suggest that the up-regulation of TREK-2 and TRESK by progesterone may contribute to the regulation of physiological changes during pregnancy.

Keywords: endometrium, Korean cattle, progesterone, two-pore domain K$^+$ channel

Article

Journal of Embryo Transfer 2011; 26(3): 209-214

Published online September 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

임신 자궁 내막에서 Two-pore Domain 칼륨 통로의 발현 변화

최창용, 탁현민, 김창운, 한재희, 강다원

농촌진흥청 국립축산과학원, 경상대학교 의학전문대학원 생리학교실/건강과학연구원, 성균관대학교 삼성창원병원, 경상대학교 의학전문대학원 생리학교실/건강과학연구원, 경상대학교 의학전문대학원 생리학교실/건강과학연구원

Alteration in Two-pore Domain K$^+$ Channel Expression in Endometrium of Pregnant Korean Cattle

최창용, 탁현민, 김창운, 한재희, 강다원

농촌진흥청 국립축산과학원, 경상대학교 의학전문대학원 생리학교실/건강과학연구원, 성균관대학교 삼성창원병원

Abstract

Endometrium undergoing hormonal change plays important roles in preparation for implantation, fetal growth, and well-being. During pregnancy, cellular remodeling and hormonal changes in endometrium could change two-pore domain K$^+$ channel (K$_{2P}$) expression. This study was performed to identify whether K$_{2P}$ channel expression is changed in endometrium of pregnant Korean cattle, and whether the expression level is modulated by progesterone treatment. We investigated changes in the mRNA and protein expressions of K$_{2P}$ channel in pregnant endometrium using RT-PCR and Western blot analyses. The expression levels of all K$_{2P}$ channel mRNAs tested in this study, except that of TREK-1, were changed in the pregnant endometrium. mRNA levels of TASK-3 and TRAAK were significantly down-regulated, whereas those of TREK-2 and TRESK were up-regulated in the pregnant endometrium. In parallel with the RT-PCR results, Western blot analysis revealed up-regulations of TREK-2 (7.9-fold) and TRESK (2-fold) proteins levels in the pregnant endometrium. In addition, TREK-2 and TRESK protein levels were up-regulated in bovine endometrial cells by progesterone treatment (10 ${mu}g$/ml). From these results, we suggest that the up-regulation of TREK-2 and TRESK by progesterone may contribute to the regulation of physiological changes during pregnancy.

Keywords: endometrium, Korean cattle, progesterone, two-pore domain K$^+$ channel