JARB Journal of Animal Reproduction and Biotehnology

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Journal of Embryo Transfer 2012; 27(4): 193-203

Published online December 31, 2012

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Factors Affecting the Development of Embryos Produced by Nuclear Transfer

Lee, Joon-Hee, Campbell, Keith H.S.

Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea, Animal Developmental and Biotechnology Group, Department of Animal Science, School of Biosciences, The University of Nottingham, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, United Kingdom , nstitute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea

Abstract

The development of embryos reconstructed by nuclear transfer is dependent upon numerous factors including the type of recipient cell, method of enucleation, the type of donor cell, method of reconstruction, activation, the cell cycle stage of both the donor nucleus and the recipient cytoplasm and the method of culture of the reconstructed embryos. Many of these points which have been reviewed extensively elsewhere (Sun and Moor, 1995; Colman, 1999; Oback and Wells, 2002; Renard et al., 2002; Galli et al., 2003b), here we will concentrate on main area, the production of suitable cytoplast and nuclear donor, nuclear-cytoplasmic coordination, oocyte activation, culture of reconstructed embryos, and the effects that this may have on development.

Keywords: nuclear transfer, cytoplast, nuclear donor cell, nuclear-cytoplasmic coordination, oocyte activation

Article

Journal of Embryo Transfer 2012; 27(4): 193-203

Published online December 31, 2012

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Factors Affecting the Development of Embryos Produced by Nuclear Transfer

Lee, Joon-Hee, Campbell, Keith H.S.

Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 600-701, Republic of Korea, Animal Developmental and Biotechnology Group, Department of Animal Science, School of Biosciences, The University of Nottingham, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, United Kingdom , nstitute of Agriculture & Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Korea

Abstract

The development of embryos reconstructed by nuclear transfer is dependent upon numerous factors including the type of recipient cell, method of enucleation, the type of donor cell, method of reconstruction, activation, the cell cycle stage of both the donor nucleus and the recipient cytoplasm and the method of culture of the reconstructed embryos. Many of these points which have been reviewed extensively elsewhere (Sun and Moor, 1995; Colman, 1999; Oback and Wells, 2002; Renard et al., 2002; Galli et al., 2003b), here we will concentrate on main area, the production of suitable cytoplast and nuclear donor, nuclear-cytoplasmic coordination, oocyte activation, culture of reconstructed embryos, and the effects that this may have on development.

Keywords: nuclear transfer, cytoplast, nuclear donor cell, nuclear-cytoplasmic coordination, oocyte activation