JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Journal of Embryo Transfer 2013; 28(1): 57-61

Published online March 31, 2013

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Microtubule-associated Protein 1B Interacts with Glyceraldehydes 3-Phosphate Dehydrogenase in Bovine Follicles

최창용, 한재희, 강다원

경상대학교 의학전문대학원 생리학교실,건강과학연구원, 농촌진흥청 국립축산과학원

Abstract

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

Keywords: follicle, GAPDH, Korean cattle, MAP1B

Article

Journal of Embryo Transfer 2013; 28(1): 57-61

Published online March 31, 2013

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

한우 난포에서 발현하는 MAP1B와 GAPDH의 상호 결합

최창용, 한재희, 강다원

농촌진흥청 국립축산과학원, 경상대학교 의학전문대학원 생리학교실.건강과학연구원, 경상대학교 의학전문대학원 생리학교실.건강과학연구원

Microtubule-associated Protein 1B Interacts with Glyceraldehydes 3-Phosphate Dehydrogenase in Bovine Follicles

최창용, 한재희, 강다원

경상대학교 의학전문대학원 생리학교실,건강과학연구원, 농촌진흥청 국립축산과학원

Abstract

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

Keywords: follicle, GAPDH, Korean cattle, MAP1B