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Journal of Embryo Transfer -0001; 28(4): 315-318

Published online November 30, -0001

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

## Hormonal Changes of Serum Progesterone and Estradiol-$17{eta}$ on Flushing Feed before Estrus during Estrous Cycle in Sows of Landrace, Yorkshire and $F_1$ (Landrace ${imes}$ Yorkshire)

Lee, Mi-Jin, Choi, Sun-Ho, Sa, Soo-Jin, Lee, Kyoung-Mi, Lee, So-Young, Cho, Kyu-Ho, Hong, Jun-Ki, Kim, Du-Wan, Kim, Ki-Hyun, Park, Jun-Cheol

National Institute of Animal Science, RDA, Cheonan 330-801, Korea

### Abstract

This study was conducted to investigate the changes of hormone levels of serum progesterone (P4) and estradiol-$17{eta}$ (E2) in sows of Landrace (L), Yorkshire (Y) and F1 (L ${ imes}$ Y) (respectively n=3) with excellent ability, and to provide a baseline data for improving reproductive performance. In this experiment, the sows at the age of 12 months or more were used. The sows were fed by two way methods, one is conventional methods and the other is 3 days-flushing feed before estrus. Each pig's blood was collected in 3, 6, 9, 12, 15 and 18 days after the estrus for the analyses of P4 and E2. Serum was separated by centrifugation for 15 min. with 3,000 rpm. Progesterone and estradiol-$17{eta}$ were measured by immunochemical assay (ELIZA test). In conventional feeding, serum progesterone levels were significantly (p<0.01) higher in F1 than in L and Y. No significant differences in P4 concentrations were seen between the L and Y of sows. Serum E2 levels were similar the serum progesterone levels. In the case of flushing feed, the tendency of hormonal changes were similar to conventional methods. But almost of hormonal levels were a little higher than that of conventional methods. P4 level of L and Y in flushing feed were significantly different (p<0.01). Serum E2 level of Y in flushing feed was significantly different among the breeds (p<0.01). These results were similar to the tendency of hormonal changes in general sows and moreover, flushing feed is known to develop the swine production, these results proved the fact of the methods. And these results suggested that more studies about hormonal changes in sows according to seasonal and nutritional factors should be needed.

Keywords: sow, Landrace, Yorkshire, progesterone, estradiol-$17{eta}$

### Article

Journal of Embryo Transfer -0001; 28(4): 315-318

Published online November 30, -0001

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

## Hormonal Changes of Serum Progesterone and Estradiol-$17{eta}$ on Flushing Feed before Estrus during Estrous Cycle in Sows of Landrace, Yorkshire and $F_1$ (Landrace ${imes}$ Yorkshire)

Lee, Mi-Jin, Choi, Sun-Ho, Sa, Soo-Jin, Lee, Kyoung-Mi, Lee, So-Young, Cho, Kyu-Ho, Hong, Jun-Ki, Kim, Du-Wan, Kim, Ki-Hyun, Park, Jun-Cheol

National Institute of Animal Science, RDA, Cheonan 330-801, Korea

### Abstract

This study was conducted to investigate the changes of hormone levels of serum progesterone (P4) and estradiol-$17{eta}$ (E2) in sows of Landrace (L), Yorkshire (Y) and F1 (L ${ imes}$ Y) (respectively n=3) with excellent ability, and to provide a baseline data for improving reproductive performance. In this experiment, the sows at the age of 12 months or more were used. The sows were fed by two way methods, one is conventional methods and the other is 3 days-flushing feed before estrus. Each pig's blood was collected in 3, 6, 9, 12, 15 and 18 days after the estrus for the analyses of P4 and E2. Serum was separated by centrifugation for 15 min. with 3,000 rpm. Progesterone and estradiol-$17{eta}$ were measured by immunochemical assay (ELIZA test). In conventional feeding, serum progesterone levels were significantly (p<0.01) higher in F1 than in L and Y. No significant differences in P4 concentrations were seen between the L and Y of sows. Serum E2 levels were similar the serum progesterone levels. In the case of flushing feed, the tendency of hormonal changes were similar to conventional methods. But almost of hormonal levels were a little higher than that of conventional methods. P4 level of L and Y in flushing feed were significantly different (p<0.01). Serum E2 level of Y in flushing feed was significantly different among the breeds (p<0.01). These results were similar to the tendency of hormonal changes in general sows and moreover, flushing feed is known to develop the swine production, these results proved the fact of the methods. And these results suggested that more studies about hormonal changes in sows according to seasonal and nutritional factors should be needed.

Keywords: sow, Landrace, Yorkshire, progesterone, estradiol-$17{eta}$

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