JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

Article Search

Article
Split Viewer

Reproductive & Developmental Biology 2010; 34(3): 253-256

Published online September 30, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

진도개 정액의 연령별 성상 및 동결성에 관한 연구

최선호,김성재,조상래,최창용,손준규,김종석,오석일,박병빈,김상현

농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,진도개사업소,진도개사업소,진도개사업소,진도개사업소

Abstract

This study was performed to investigate the characteristics within ages and freezing tolerance of spermatozoa in Jindo Dog. Experimental animals were selected 12 herds within 1~8 year’s old and collected semen for 2 times in a week. Collected semen was evaluated whole volume and sperm number with CASA system (SIAS, Medical Supply, Korea). Then seminal plasma were separated and diluted with modified Tris-egg yolk extender and added 4, 6 and 8% glycerol for 4 times to final concentration and equilibrated for 1.5 hrs. Before and after freezing, equilibrated semen were evaluated the survival rates. Total volume of sperm at 1~2 year old group is as 5.2×108 cells/ ml largest and there were no significance among groups. The motility of 1~2 year old group is highest as 90.9% and there were significance among groups. Abnormal sperm showed similar among groups. The survival rate in terms of pre-freezing and post-freezing were decreased all levels of glycerol and reveled 87.0% to 64.5% in 4%, 87.5% to 51.9% in 6% and 73.4% to 29.7% in 8%, there were significant difference among the groups (p<0.05). These results suggest that the optimal sperm-freezing methods in Jindo Dog are utilized with modified Tris egg-yolk extender with 4% glycerol and were improve the reproductive activity by these methods.

Keywords: Jindo-Dog, Spermatozoa, Age, Freezing, Glycerol

Article

Reproductive & Developmental Biology 2010; 34(3): 253-256

Published online September 30, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

진도개 정액의 연령별 성상 및 동결성에 관한 연구

최선호,김성재,조상래,최창용,손준규,김종석,오석일,박병빈,김상현

농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,농촌진흥청 국립축산과학원,진도개사업소,진도개사업소,진도개사업소,진도개사업소

Abstract

This study was performed to investigate the characteristics within ages and freezing tolerance of spermatozoa in Jindo Dog. Experimental animals were selected 12 herds within 1~8 year’s old and collected semen for 2 times in a week. Collected semen was evaluated whole volume and sperm number with CASA system (SIAS, Medical Supply, Korea). Then seminal plasma were separated and diluted with modified Tris-egg yolk extender and added 4, 6 and 8% glycerol for 4 times to final concentration and equilibrated for 1.5 hrs. Before and after freezing, equilibrated semen were evaluated the survival rates. Total volume of sperm at 1~2 year old group is as 5.2×108 cells/ ml largest and there were no significance among groups. The motility of 1~2 year old group is highest as 90.9% and there were significance among groups. Abnormal sperm showed similar among groups. The survival rate in terms of pre-freezing and post-freezing were decreased all levels of glycerol and reveled 87.0% to 64.5% in 4%, 87.5% to 51.9% in 6% and 73.4% to 29.7% in 8%, there were significant difference among the groups (p<0.05). These results suggest that the optimal sperm-freezing methods in Jindo Dog are utilized with modified Tris egg-yolk extender with 4% glycerol and were improve the reproductive activity by these methods.

Keywords: Jindo-Dog, Spermatozoa, Age, Freezing, Glycerol

JARB Journal of Animal Reproduction and Biotehnology

qr code

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663