JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Reproductive & Developmental Biology 2011; 35(3): 215-219

Published online September 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

프로게스테론과 에스트라디올 17β에 의한 Long-ChainAcyl-CoA Synthetase4의 발현 조절

박효영,강만종

전남대학교 농업생명과학대학 동물자원학부

Abstract

Acyl-CoA synthetase 4(ACSL4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues and postulated to modulate eicosanoid production. The human and mouse ACSL4 gene are mapped on chromosome X. The female mice heterozygous for ACSL4 deficiency became pregnant less frequently and produced small litters, with 40% of embryos surviving gestation. In this study, we examined the regulation of ACS4 by estradiol-17β and progesterone (P4) in the human endometrial cancer cell line HTB-1B. ACSL4 mRNA was increased in a dose-dependent manner. Also, expression of ACSL4 gene was up-regulated in a time-dependent manner in HTB-1B cells. However, combined treatment with progesterone and estradiol-17β modestly decreased the levels of ACS4L mRNA as compared with the estradiol-17β and progesterone respectively. Overall, these results suggest that the ACSL4 gene is regulated by progesterone and estradiol-17β in the HTB-1B cells.

Keywords: Acyl-CoA synthetase, Pregnancy, Estradiol-17β (E2), Progesterone (P4), Implantation

Article

Reproductive & Developmental Biology 2011; 35(3): 215-219

Published online September 30, 2011

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

프로게스테론과 에스트라디올 17β에 의한 Long-ChainAcyl-CoA Synthetase4의 발현 조절

박효영,강만종

전남대학교 농업생명과학대학 동물자원학부

Abstract

Acyl-CoA synthetase 4(ACSL4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues and postulated to modulate eicosanoid production. The human and mouse ACSL4 gene are mapped on chromosome X. The female mice heterozygous for ACSL4 deficiency became pregnant less frequently and produced small litters, with 40% of embryos surviving gestation. In this study, we examined the regulation of ACS4 by estradiol-17β and progesterone (P4) in the human endometrial cancer cell line HTB-1B. ACSL4 mRNA was increased in a dose-dependent manner. Also, expression of ACSL4 gene was up-regulated in a time-dependent manner in HTB-1B cells. However, combined treatment with progesterone and estradiol-17β modestly decreased the levels of ACS4L mRNA as compared with the estradiol-17β and progesterone respectively. Overall, these results suggest that the ACSL4 gene is regulated by progesterone and estradiol-17β in the HTB-1B cells.

Keywords: Acyl-CoA synthetase, Pregnancy, Estradiol-17β (E2), Progesterone (P4), Implantation