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Reproductive & Developmental Biology 2013; 37(4): 255-261
Published online December 30, 2013
Copyright © The Korean Society of Animal Reproduction and Biotechnology.
A Proteome Reference Map for Porcine Plasma Proteins
Jin Young Jeong,Jin Sun Nam,Mi Rim Park,Jang Mi Kim,Hak Jae Jeong,Kyung Woon Kim,Hyun-Jeong Lee
Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration,Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration
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To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.
Keywords: 2-DE, Proteome, Porcine, MALDI-TOF-TOF, LC-MS-MS
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Reproductive & Developmental Biology 2013; 37(4): 255-261
Published online December 30, 2013
Copyright © The Korean Society of Animal Reproduction and Biotechnology.
A Proteome Reference Map for Porcine Plasma Proteins
Jin Young Jeong,Jin Sun Nam,Mi Rim Park,Jang Mi Kim,Hak Jae Jeong,Kyung Woon Kim,Hyun-Jeong Lee
Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration,Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration,Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration,Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration
Abstract
To profile the proteome in porcine plasma, blood samples were collected from adult male barrows and those plasma were retrieved. For the depletion or pre-fractionation of high-abundance proteins, plasma samples were treated with commercial kits. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than forty six proteins were identified and the reference map was constructed. The pre-treatment for the removal of high-abundance proteins caused the changes in 2-DE images and some of the proteins were newly uncovered after the most of high abundant proteins were removed. However, it is expected for further steps necessary to identify more low-abundance proteins that may contain potential bio-markers.
Keywords: 2-DE, Proteome, Porcine, MALDI-TOF-TOF, LC-MS-MS
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